Nejire Hado enrolled in U.A. High School where she met Mirio Togata and Tamaki Amajiki. She befriended them, and over time, their skills and abilities with their Quirks made them known as The Big 3, the most powerful students at school. After receiving her Provisional Hero License, she started working for the Dragoon Hero: Ryukyu. At another point, Nejire participated in her second year Beauty Pageant during the U.A. School Festival, but was defeated by Bibimi Kenranzaki.
Nejire is first seen at the assembly where Vlad King and Hound Dog announce Katsuki and Izuku's punishment for fighting after hours in Ground Beta. She asks Mirio Togata if he knows who the students involved are, though he doesn't respond and merely becomes excited at hearing of such students.
She is later invited along with the other Big 3 to speak with Class 1-A by Eraser Head as part of their introduction to Hero Internships. With Tamaki turning his back to the class because of stage fright, Nejire begins introducing herself and why she and the rest of the Big 3 are present. She immediately begins asking questions of the students rapidly, not allowing them time to answer before she asks more.
When Mirio challenges all of Class 1-A to face him at once, she is present and tells everyone about a student who got so frustrated that they quit wanting to become a hero, advising Mirio to be cautious so as not to have a repeat of that incident, playing with Mina's horns all the while as she recounts this tale. After Mirio easily defeats Class 1-A Nejire enthusiastically offers to explain Mirio's Quirk to everyone, however, Tamaki tells her it is Mirio's turn to speak and she pouts at the latter as he explains to the class whilst apologizing to her.
During the Internships, she helps out Ochaco and Tsuyu, recommending them to the hero she was interning with, Ryukyu. She takes out two opposing gang members with Gigantification Quirks in one move and also coordinates a Super Move with the other two girls to completely quell all the villains they were facing. Ryukyu notes that Nejire's timing when going for the knockout blow has improved and praises Tsuyu and Ochaco for having such great aptitude.
When a meeting is called to help organize the Shie Hassaikai Raid, Nejire is also invited as Ryukyu's Intern and rushes to hug her teacher when she spots her. During the meeting, she explains to Tsuyu and Ochaco what the "Hero Network" is, an internet service that people with a Hero License can use, which she had heard allowed Heroes to see activity reports and apply for help from other Heroes with useful Quirks throughout the country.
After the meeting concludes she and Tamaki console Mirio after he was devastated to learn that Eri was used to make the Quirk-destroying bullets and tell him to look forward and do his best to save her.
For several days, students wait patiently to receive any news of Eri's whereabouts. One day, late at night, the Big 3 gather after receiving a message containing information, 'on the day carry out the plan to raid Shie Hassaikai'. The next day, all the members of the Raid team wake up early as the day of the operation dawns and Nejire is present with everyone else as they prepare to begin.
As everyone gathers, Tsuyu notes that this is something they are all unfamiliar with and Nejire agrees, saying all of it was very out there whilst also pointing out that Tsuyu liked to put her finger on her chin a lot. As part of the Ryukyu group, she leads Tsuyu and Ochaco into supporting Ryukyu as she took on the Yakuza's first line of defense outside their hideout.
Whilst the other teams battle inside, Nejire and the rest of Ryukyu's team fight Rikiya Katsukame of the Eight Bullets. They eventually manage to overpower him and Ryukyu orders the Police Force to restrain Rikiya while Nejire, Ochaco, and Tsuyu prepare to head after Sir Nighteye's group.
Suddenly, Ochaco and Tsuyu along with the Police Force become tired due to Rikiya inhaling their stamina, much to Ryukyu's surprise since she thought she had knocked him out. Conscious, Rikiya reveals that he took a drug earlier and it is beginning to take effect, which enhances his Quirk and allows him to absorb a target's stamina simply by inhaling.
Now larger and stronger than before, Rikiya attacks the Police Force only for Ryukyu to take them to safety. 20 minutes pass and Ryukyu's group is in a standstill against the rejuvenated Rikiya. Nejire decides to take action and attacks Rikiya with her Quirk, which allows her to convert her own stamina into pure energy and emit shockwaves, although Rikiya manages to block her attack.
When Rikiya's drug wears off, he demands Nejire to let him touch her, which she vehemently refuses. Ryukyu drags Rikiya to the crossroads and commands Ochaco to make them float and Tsuyu to help drag them towards the crossroad. With Ryukyu and Rikiya's bodies now weightless, Tsuyu drags them to the crossroad with her tongue.
At the crossroad, Ryukyu orders Nejire to blast her and Rikiya onto the ground with everything she has. Ochaco releases her Quirk's effect and Nejire blasts Ryukyu and Rikiya with her Quirk. The combined weight of Ryukyu and Rikiya along with the force of Nejire's shockwave is enough to make the crossroad collapse, causing Ryukyu's group along with Rikiya to fall down into the Eight Precepts of Death's underground where they land in the middle of the battle between Izuku and Kai Chisaki.
After the operation is successful she surveys the site for damage and is impressed when she notices that Izuku had managed to minimize damage by keeping Overhaul pinned to one spot.
With the beginning of October, she and the rest of the Work Studies are allowed to go to Sir Nighteye's funeral, along with the Big Three, All Might, and Shota. Due to what happened, they are informed that the Hero Work Studies program is postponed for the moment, so Nejire, Ochaco, and Tsuyu say goodbye to Ryukyu.
Nejire decides to compete in the Miss Con Beauty Pageant of the U.A. School Festival, she had entered the pageant for the previous two years as well but failed to win either, losing out to Bibimi Kenranzaki of the Support Course in the preceding year. When Eri comes to visit U.A. with Mirio and Shota, she meets with Nejire as well and finds her busy in preparation for the pageant, as Tamaki posits that Nejire's loss last year was because her appeal is more on the "cute" side and she tried to compete with Bibimi in terms of "flashiness" instead.
He also mentions that later in the year Nejire, Bibimi, and Itsuka Kendo are scheduled to shoot a commercial that is expected to boost their popularity. Yuyu Haya, Nejire's best friend, is also present helping around with preparations for her friend's entry. Nejire flashes a smile and says she is determined to win the pageant this year as it is her last chance to do so. As the day of the Festival draws near Nejire does her best to prepare for the pageant, with help from Yuyu.
On the day of the Festival, Nejire overhears Neito Monoma telling Itsuka that there is no doubt she will win. Nejire interjects and says that they still don't know for sure and wishes Itsuka the best of luck. At that moment, Bibimi arrives and says she is the only one blessed with flawless beauty, feeling assured of her victory.
When the Miss Con beauty pageant finally gets underway Itsuka goes first with a karate display, followed by Bibimi who brings in a tank of her own invention in the shape of her own face. After them is Nejire's turn and she begins a stunning display that plays to her strengths, using her Quirk to fly around and do a dance in mid-air, captivating the audience.
After voting is done and the results are announced, Nejire is chosen as the winner. Later on, when Izuku is told that U.A. has been put in charge of looking after Eri, Nejire is present with the rest of the Big 3 and does Eri's hair for her.
The Big 3 are taking care of Eri in her room when she suddenly starts crying, saying that her horn's started to feel unusual. While Mirio and Tamaki hurry off to look for Shota, Nejire stays with Eri, hugging and comforting her. Shota arrives and erases Eri's Quirk, gently patting her on the head and telling her not to worry.
The months go by until it's late March. Thanks to the police investigation and its spy Hawks, the Hero Public Safety Commission has found out the plans of the Paranormal Liberation Front and its allies. With this info, the Commission organized a large force of Pro Heroes, divided into several teams to take down the villainous organization.
A team of heroes has the mission of storming the main headquarters of the Paranormal Liberation Front, while another team lead by Endeavor will do the same at the Jaku General Hospital, where the Nomu are created. Finally, a third team, made up mainly of students and lead by Burnin, will be in charge of evacuating the residents of a city near the hospital and taking them to a safe place. Nejire is in the last one. When everything is ready, the operation starts as Burnin signals the students to start evacuating the citizens. 
The evacuation of the city proceeds without any problem. Nejire is in charge of flying over the city to ensure that all citizens are being evacuated. Suddenly, the hospital and the entire area around it begin to falling apart, and the disintegration wave spreads rapidly until it reaches the city itself.
Realizing that destruction does not stop, Burnin quickly orders everyone to run away as fast as possible while evacuating the civilians who are still there as best they can. Nejire uses her Wave Motion Quirk to propel multiple vehicles away from danger.
As Gigantomachia started approaching Jakku City, the Heroes were rallied up to stop the giant, while Nejire and Tenya Ida went to the site of battle with Tomura to help the Heroes out. She manages to catch the villain off-guard and blast him with a wave of energy. She notices the beaten-up Ryukyu and decides that, together with Shoto, they will take Tomura down. They unleash their Super Moves on Tomura, but before they can defeat him, Gigantomachia intercepts his master and saves him from certain death.
Suddenly, Best Jeanist appeared and restrained both Gigantomachia and the League. Spinner then tried to wake his leader up, so he could give the giant an order, but Nejire appeared and was about to blast them off with her Quirk. However, Dabi used his Quirk, scorching her left side of the body and blasting her away. She was caught by Ingenium before she could hit the ground. Thankfully she recovers upon Lemillion's sudden arrival on the battlefield and continues to fight alongside the other students. She hears Katsuki announce his Hero name as "Great Explosion Murder God Dynamight" and says it sounds disturbing.
Nejire continues fighting the Near High-End Nomus, until "Tomura" wakes-up and uses his Radio Waves Quirk to summon all the Nomu to get him out of there. Nejire and the rest of the heroes who can still fight try to stop them, but "Tomura" manages to escape with a handful of allies.